Coding

Part:BBa_K2012009:Design

Designed by: Jun Li   Group: iGEM16_HZAU-China   (2016-09-14)


RBS(J61100) and CheZ with fused with a GS linker and GFP in C teminal


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1321


Design Notes

CheZ-GSlinker(1*GGGGS)-GFP


Source

GFP is from BBa.E0034 and CheZ is amplify from E.coli's Genome. GSlinker is GGGGS，4 anino acid,which in DNA form is 15bp.


References